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Registros recuperados: 255
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Caracterização molecular de dois isolados brasileiros de Lettuce mosaic virus apresentando propriedades biológicas distintas Trop. Plant Pathol.
KRAUSE-SAKATE,RENATE; MELLO,RAQUEL N.; PAVAN,MARCELO A.; ZAMBOLIM,EUNIZE M.; CARVALHO,MURILO G.; LE GALL,OLIVIER; ZERBINI,F. MURILO.
Efetuou-se a clonagem e seqüenciamento do gene que codifica a proteína capsidial de dois isolados do vírus do mosaico da alface (Lettuce mosaic virus, LMV) provenientes do estado de São Paulo, previamente caracterizados como pertencentes aos patótipos II (AF198, incapaz de infetar cultivares com os genes de resistência mo1¹ ou mo1²) e IV (AF199, capaz de quebrar a resistência propiciada pelos genes mo1¹ e mo1²), com base na virulência em cultivares diferenciadoras. Análise comparativa das seqüências de nucleotídeos de isolados provenientes da Europa, América do Norte, Oriente Médio e os dois isolados brasileiros não permitiu sua separação em estirpes, pois as porcentagens de homologia foram sempre superiores a 95%. Entretanto, análise filogenética dos...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lactuca; Lettuce; Potyvirus; LMV; Capsid protein; Cloning; Resistance.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582001000200006
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Carbapenem-resistant Pseudomonas aeruginosa: association with virulence genes and biofilm formation BJM
Gonçalves,Iara Rossi; Dantas,Raquel Cristina Cavalcanti; Ferreira,Melina Lorraine; Batistão,Deivid William da Fonseca; Gontijo-Filho,Paulo Pinto; Ribas,Rosineide Marques.
Abstract Pseudomonas aeruginosa is an opportunistic pathogen that causes frequently nosocomial infections, currently becoming more difficult to treat due to the various resistance mechanisms and different virulence factors. The purpose of this study was to determine the risk factors independently associated with the development of bacteremia by carbapenem-resistant P. aeruginosa, the frequency of virulence genes in metallo-β-lactamases producers and to evaluate their ability to produce biofilm. We conducted a case–control study in the Uberlândia Federal University – Hospital Clinic, Brazil. Polymerase Chain Reaction was performed for metallo-β-lactamases and virulence genes. Adhesion and biofilm assays were done by quantitative tests. Among the 157 strains...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas aeruginosa; Resistance; Metallo-β-lactamases; Virulence; Biofilm.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000200211
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Carriage frequency, phenotypic and genotypic characteristics of Staphylococcus aureus isolated from dialysis and kidney tranplant patients at a hosptial in northern Paraná BJM
Giarola,Luciana Borges; Santos,Rosiane Ribeiro dos; Tognim,Maria Cristina Bronharo; Borelli,Sueli Donizete; Bedendo,João.
The objective of the present study was to determine the frequency of Staphylococcus aureus nasal carriage among dialysis and kidney transplant patients, to identify the antimicrobial resistance profile of these strains and to verify their genetic profiles with the RW3A primer. The study included 159 individuals, comprising 111 dialysis and 48 kidney transplant patients. Of the 48 transplant patients, 75% were positive for S. aureus, whereas 49% of the 111 dialysis patients were carriers. Two samples yielded conflicting results for oxacillin sensitivity between the disk diffusion and minimum inhibitory concentration (MIC) assays: both were sensitive by the disk diffusion assay and resistant by MIC (4 μg/ml). In the antibiogram by disk diffusion, ten samples...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Staphylococcus aureus; Nasal carrier; Resistance; MecA gene; Genetic typing.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300011
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Changes in antimicrobial susceptibility of commonly clinically significant isolates before and after the interventions on surgical prophylactic antibiotics (SPAs) in Shanghai BJM
Wang,Su; Han,Li-Zhong; Ni,Yu-Xing; Zhang,Yi-Bo; Wang,Qun; Shi,Da-Ke; Li,Wen-Hui; Wang,Yi-Chen; Mi,Chen-Rong.
Abstract Surveillances and interventions on antibiotics use have been suggested to improve serious drug-resistance worldwide. Since 2007, our hospital have proposed many measures for regulating surgical prophylactic antibiotics (carbapenems, third gen. cephalosporins, vancomycin, etc.) prescribing practices, like formulary restriction or replacement for surgical prophylactic antibiotics and timely feedback. To assess the impacts on drug-resistance after interventions, we enrolled infected patients in 2006 (pre-intervention period) and 2014 (post-intervention period) in a tertiary hospital in Shanghai. Proportions of targeted pathogens were analyzed: methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant Enterococcus spp. (VRE),...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Surgical prophylactic antibiotics; Intervention; Resistance; Nosocomial infection.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000300552
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Characterization of Mycobacterium tuberculosis complex isolated from iranian and afghani patients by spoligotyping method BJM
Ramazanzadeh,Rashid; Farnia,Parisa; Amirmozafari,Nour.
Designing newer drugs, vaccines, and diagnostic techniques is dependent on better understanding of M. tuberculosis virulence mechanism. In this study the prevalence of pcaA gene was determined in M. tuberculosis strains typed by spoligotyping. The associated risk factors among patients with different nationalities residing in Iran were also determined. The isolated M. tuberculosis strains have been characterized by performing susceptibility tests against four first-line antituberculosis drugs and were then subjected to spoligotyping characterization. PCR was used for detection of pcaA gene and its nucleotide sequence was also determined. Spoligotyping of M. tuberculosis strains resulted in 140 different patterns. One hundred twenty two (87.1%) of these...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tuberculosis; Resistance; Drugs; Spoligotyping; PcaA.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200019
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Chloroquine resistance status a decade after : Re-emergence of sensitive Plasmodium falciparum strains in malaria endemic and epidemic areas in Kenya OAK
Wangai, L.N; Kimani, F.T; Omar, S.A; Karanja, S.M; Nderu, D.W; Magoma, G; Mutua, D.
Development and spread of chloroquine (CQ) resistance led to its withdrawal in most malaria endemic countries. In Kenya, this occurred in 1998 when clinical efficacy dropped below 50%. Less than a decade after CQ was removed from routine use in Malawi, the drug has reversed to activity and is again effective for first-line treatment of uncomplicated malaria. There is a probability of a similar reversed activity in Kenya for more 10 years of its absence in uncomplicated Plasmodium falciparum malaria treatment. The present study was aimed at establishing the CQ resistance status in the country, 10 years after its withdrawal, by looking at high malaria transmission zone, Mbita, a malaria endemic area and some malaria epidemic areas of the Kenyan highlands....
Palavras-chave: Plasmodium falciparum; Chloroquine (CQ); Resistance; Endemic; Epidemic.
Ano: 2011 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3096
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Clinical failure of vancomycin treatment of Staphylococcus aureus infection in a tertiary care hospital in southern Brazil BJID
Lutz,Larissa; Machado,Adão; Kuplich,Nadia; Barth,Afonso Luís.
We describe a case of clinical failure of vancomycin treatment of Staphylococcus aureus infection and the laboratory characteristics of the organism in a tertiary referral university hospital in southern Brazil. An 11-month-old male patient presented with pneumonia and S. aureus was isolated from his respiratory tract. Initial treatment with oxacillin and gentamicin was ineffective. Vancomycin was added to the regimen as the patient worsened, but after the 30th day of vancomycin treatment S. aureus was isolated from the blood. This isolate had a minimum inhibitory concentration (MIC) for vancomycin of 4 µg/mL. After pre-incubation with vancomycin the isolate displayed an increase in the expression of vancomycin resistance and colonies grew in the presence...
Tipo: Info:eu-repo/semantics/report Palavras-chave: S. aureus; Resistance; Vancomycin.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702003000300008
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Coffee resistance to the main diseases: leaf rust and coffee berry disease Braz. J. Plant Physiol.
Silva,Maria do Céu; Várzea,Victor; Guerra-Guimarães,Leonor; Azinheira,Helena Gil; Fernandez,Diana; Petitot,Anne-Sophie; Bertrand,Benoit; Lashermes,Philippe; Nicole,Michel.
Considerable success has been obtained in the use of classical breeding to control economically important plant diseases, such as the coffee leaf rust and the coffee berry disease (CBD). There is a strong consensus that growing genetically resistant varieties is the most appropriate cost effective means of managing plant diseases and is one of the key components of crop improvement. It has also been recognized that a better knowledge of both, the pathogens and the plant defence mechanisms will allow the development of novel approaches to enhance the durability of resistance. After a brief description of concepts in the field of plant disease resistance, we attempt to give a view of the research progress on coffee leaf rust and CBD concerned with the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coffea; Colletotrichum kahawae; Hemileia vastatrix; Coffee breeding; Resistance.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000100010
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Collateral methotrexate resistance in cisplatin-selected murine leukemia cells BJMBR
Bhushan,A.; Hacker,M.P.; Tritton,T.R..
Resistance to anticancer drugs is a major cause of failure of many therapeutic protocols. A variety of mechanisms have been proposed to explain this phenomenon. The exact mechanism depends upon the drug of interest as well as the tumor type treated. While studying a cell line selected for its resistance to cisplatin we noted that the cells expressed a >25,000-fold collateral resistance to methotrexate. Given the magnitude of this resistance we elected to investigate this intriguing collateral resistance. From a series of investigations we have identified an alteration in a membrane protein of the resistant cell as compared to the sensitive cells that could be the primary mechanism of resistance. Our studies reviewed here indicate decreased tyrosine...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Methotrexate transport; Resistance; Cisplatin; Leukemia; Phosphorylation.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000700005
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Commercial sugar beet cultivars evaluated for rhizomania resistance and storability in Idaho, 2014 NWISRL
Strausbaugh, C.A.; Eujayl, Imad A.; Wambolt, C.
Rhizomania caused by Beet necrotic yellow vein virus (BNYVV) and storage losses are serious sugar beet production problems. To identify sugar beet cultivars with resistance to BNYVV and evaluate storability, 33 commercial cultivars were screened by growing them in a sugar beet field infested with BNYVV in Kimberly, ID during the 2014 growing season in a randomized complete block design with 6 replications. At harvest on 24-25 September 2014, roots were dug and evaluated for symptoms of rhizomania and also placed in an indoor commercial sugar beet storage building. After 138 days in storage, samples were evaluated for surface rot, weight loss, and sucrose loss. Surface root rot ranged from 7 to 82%, weight loss ranged from 9.4 to 19.1%, sucrose losses...
Tipo: Article Palavras-chave: Resistance; Rhizomania; Sugarbeet.
Ano: 2015 URL: http://eprints.nwisrl.ars.usda.gov/1602/1/1560.pdf
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Community-associated methicillin-resistant Staphylococcus aureus in non-outbreak skin infections BJM
Bonesso,Mariana Fávero; Marques,Silvio Alencar; Camargo,Carlos Henrique; Fortaleza,Carlos Magno Castelo Branco; Cunha,Maria de Lourdes Ribeiro de Souza da.
The aim of this study was to determine the prevalence of Staphylococcus aureus and risk factors for the acquisition of MRSA (Methicillin Resistant Staphylococcus aureus) as the main cause of skin and soft tissue infections. S. aureus were characterized for the presence of PVL, TSST-1 and mecA genes. SCCmec typing was carried out in mecA positive strains and PFGE was performed only in these strains. During the study period, 127 outpatients attending a dermatology clinical the Botucatu Medical School, a regional tertiary hospital in Botucatu, Sao Paulo, Brazil, were diagnosed with active skin infections. A total 66 (56.9%) S. aureus strains were isolated. The methicillin resistance gene mecA was detected in seven (10.6%) S. aureus strains. The SCCmec types...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Staphylococcus aureus; Resistance; Skin infections; Virulence; Epidemiology.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400034
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Como o pecuarista do Semi-Árido nordestino deve enfrentar emergencialmente uma seca. Infoteca-e
GUIMARAES FILHO, C..
bitstream/CPATSA/7270/1/INT6.pdf
Tipo: Comunicado Técnico (INFOTECA-E) Palavras-chave: Brasil; Nordeste; Resistance; Northeast.; Produção animal; Pecuária; Resistência; Seca; Tecnologia.; Animal production; Drought; Livestock; Brazil; Technology..
Ano: 1999 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/132169
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Comparative Evaluation of the Effects of Binzalkonium Chloride, Iodine, Gluteraldehyde and Hydrogen Peroxide Disinfectants against Avian Salmonellae Focusing on Genotypic Resistance Pattern of the Salmonellae Serotypes toward Benzalkonium Chloride Rev. Bras. Ciênc. Avic.
Aksoy,A; El Kahlout,KEM; Yardimci,H.
ABSTRACT Using disinfectants in poultry houses is a common practice to ban the zoonotic pathogens like Salmonella. A major concern in using disinfectants is the emergence of bacteria strains that resist some disinfectants. This phenomenon is manifested in the resistance of some Salmonella serotypes against quaternary ammonium compounds. Such resistance is attributed to qacEΔ1 gene which may be possessed by some Salmonella serotypes. This work aimed to evaluate the resistance of Salmonella serotypes (S. Typhimurium, S. Infantis and S. Entiridis) against different disinfectants (benzalkonium chloride, iodine, gluteraldehyde and hydrogen peroxide). The effect of the disinfectants were evaluated by treatment of the bacteria with different concentrations...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Disinfectants; PCR; Poultry; Resistance; Salmonella.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2020000100301
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Comparison of clinical characteristics between healthcare-associated pneumonia and community-acquired pneumonia in patients admitted to secondary hospitals BJID
Lee,Jong Hoo; Kim,Yee Hyung.
BACKGROUND: Since healthcare-associated pneumonia (HCAP) is heterogeneous, clinical characteristics and outcomes are different from region to region. There can also be differences between HCAP patients hospitalized in secondary or tertiary hospitals. This study aimed to evaluate the clinical characteristics of HCAP patients admitted into secondary community hospitals. METHODS: This was a retrospective study conducted in patients with HCAP or community-acquired pneumonia (CAP) hospitalized in two secondary hospitals between March 2009 and January 2011. RESULTS: Of a total of 303 patients, 96 (31.7%) had HCAP. 42 patients (43.7%) resided in a nursing home or long-term care facility, 36 (37.5%) were hospitalized in an acute care hospital for > 2 days...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Health care; Pneumonia; Antibiotics; Mortality; Resistance.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000400002
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Compatibility Polymorphism Based on Long-Term Host-Parasite Relationships: Cross Talking Between Biomphalaria glabrata and the Trematode Schistosoma mansoni From Endemic Areas in Brazil ArchiMer
Lima, Mariana G.; Montresor, Lângia C.; Pontes, Joana; Augusto, Ronaldo De C.; Silva, Jairo Pinheiro Da; Thiengo, Silvana C..
Sympatric snail populations have been kept in the laboratory since the isolation of the parasite from the field. To evaluate the influence of the intermediate host in the infectivity of S. mansoni, this allopatric strain was compared to two sympatric strains, from different geographical origins, and with different time of maintenance in the laboratory. Snail–trematode compatibility was accessed for a total of nine possible combinations (three snail populations, three schistosome strains), using different charges of parasite: 1, 5, 10, and 15 miracidia/snail. Each S. mansoni strain was characterized according to its infectivity phenotype that reflects the efficiency of their infection mechanism and all B. glabrata populations were characterized according to...
Tipo: Text Palavras-chave: Snail-trematode; Host-parasite; Interaction; Compatible; Incompatible; Infectivity; Resistance; Schistosomiasis.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00489/60084/63419.pdf
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COMPETITIVE ABILITY OF WHEAT IN ASSOCIATION WITH BIOTYPES OF Raphanus raphanistrum L. RESISTANT AND SUSCEPTIBLE TO ALS-INHIBITOR HERBICIDES Ciência e Agrotecnologia
Costa,Leandro Oliveira da; Rizzardi,Mauro Antônio.
The occurrence of Raphanus raphanistrum ALS herbicide-resistant in wheat crops causes crop yield losses, which makes it necessary to understand the factors that influence the interference of this weed to develop safer management strategies. This study aimed to evaluate the competitive ability of wheat in coexistence with biotypes of R. raphanistrum that are resistant (R biotype) and susceptible (S biotypes) to ALS herbicides and to determine whether there are differences in the competitiveness of these biotypes. The experiments were conducted in a greenhouse using a completely randomized design with four replications. The treatments were placed in pots and arranged in replacement series for three experiments (1 - wheat with the R biotype; 2 - wheat with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Resistance; Competition; Replacement series.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542015000200121
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Comportamento de quatro cultivares de guar na região do Alto Sertão Pernambucano. Infoteca-e
AGUIAR FILHO, S. P. de.
Tem como objetivo conseguir culturas alternativas para o sistema produtivo dos agricultores da regiao semi-arida do Nordeste brasileiro. A EMBRAPA-CPATSA, vem desenvolvendo trabalhos de pesquisa com a cultura do guar, introduzida nessa regiao em 1974 pela Celanese do Brasil - Fibras Quimicas Ltda. Os experimentos foram instalados nos Campos Experimentais de Bebedouro (irrigado) e da Caatinga (sequeiro), no municipio de Petrolina,PE. Os solos utilizados foram classificados como latossolo unidade 37 AB para a area irrigada e Podzolico Vermelho-Amarelo Tb planossolico. A fraca textura areno-argilosa, cascalhente, fase caatinga, relevo plano, substrato muscovita xisto, na area de sequeiro.
Tipo: Comunicado Técnico (INFOTECA-E) Palavras-chave: Cultura resistente a seca; Comportamento; Cyamopsis tetragondoba; Pernambuco; Alto Sertao; Resistance; Variety; Semi-arid region.; Guar; Variedade.; Drought..
Ano: 1983 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/131305
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Contribution of in Vivo Experimental Challenges to Understanding Flat Oyster Ostrea edulis Resistance to Bonamia ostreae ArchiMer
Morga, Benjamin; Renault, Tristan; Faury, Nicole; Lerond, Sophie; Garcia, Celine; Chollet, Bruno; Joly, Jean-pierre; Lapegue, Sylvie; Harrang, Estelle; Arzul, Isabelle.
Bonamiosis due to the parasite Bonamia ostreae has been associated with massive mortality outbreaks in European flat oyster stocks in Europe. As eradication and treatment are not possible, the control of the disease mainly relies on transfer restriction. Moreover, selection has been applied to produce resistant flat oyster families, which present better survival and lower prevalence than non-selected oysters. In order to better understand the mechanisms involved in resistance to bonamiosis, cellular and molecular responses of 2 oyster groups (selected oysters and wild-type oysters) were analyzed in the context of experimental injection and cohabitation infections. Cellular responses including non-specific esterases detection, ROS production and...
Tipo: Text Palavras-chave: Bonamia ostreae; Protozoan; Ostrea edulis; Resistance; Experimental infection; Haemocytes; Gene expression; Apoptosis.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00405/51665/52213.pdf
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Control of ALS- and EPSPS-Resistant Amaranthus palmeri by Alternative Herbicides Applied in PRE- and POST-Emergence Planta Daninha
GONÇALVES NETTO,A.; NICOLAI,M.; CARVALHO,S.J.P.; MALARDO,M.R.; LÓPEZ-OVEJERO,R.F.; CHRISTOFFOLETI,P.J..
ABSTRACT: The emergence of resistant biotypes of the Amaranthus palmeri species in cotton production areas of the state of Mato Grosso, Brazil, generated the need for correct identification of this species and information on viable herbicidal tools for their management. Thus, greenhouse experiments were conducted to evaluate the efficacy of alternative herbicides applied to A. palmeri in pre and post emergence. A randomized block design with four replications was used. The efficacy of herbicides applied in pre emergence was evaluate in two experiments, one in a clayey and other in a sandy soil; 9 herbicide treatments (8 with herbicide application and a control without application) were applied on each soil. Subsequently, two experiments with different...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Caruru; Weed control; Resistance; Phenology; Soil texture.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582019000100193
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Convivência com a seca no semi-árido brasileiro. Infoteca-e
EMBRAPA. Centro de Pesquisa Agropecuária do Trópico Semi-Árido..
Aborda sobre o semi-árido brasileiro, as tecnologias disponíveis para agricultura e pecuária, o retorno econômico das principais tecnologias. Os recursos necessários, a cooperação internacional e o CPATSA.
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E) Palavras-chave: Convivência; Brasil; Nordeste; Região semi-árida; Resistance; Northeast; Semi-arid region; Natural research.; Recurso natural; Seca.; Drought; Brazil..
Ano: 1980 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/131961
Registros recuperados: 255
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